Hernia repair continues to be a problem facing surgeons today, particularly because of the high incidence of reoccurrence. This work presents preliminary data of a pioneering effort to investigate the effect of mesenchymal stromal cells (MSCs) on mechanical property enhancement in full thickness fascial defects. Heparinized MSCs harvested from a rabbit’s tibia/iliac crest were applied to two fascial defects on the rabbit’s abdominal wall, with two other defects acting as controls (no MSCs added). After an 8 week recovery period, the entire abdominal fascia was harvested for mechanical property testing and elastographic strain analysis. Preliminary results from uniaxial tensile testing indicate a significant increase in the modulus of toughness strain energy, with at least a 50% increase in the MSC treated defects as compared with the control defects. Results from the elastographic strain analysis show excellent correlation in the calibration of the elastography to the uniaxial tensile test, with nearly identical moduli of elasticity. In addition, the elastographs clearly show tissue property heterogeneity at all stages of tensile testing. The MSC treated tissue demonstrates promise of enhanced material properties over that of the nontreated tissue; testing and analysis is ongoing. The elastography provides pixel-level description of tissue property variations providing critical information on wound healing effectiveness that would be impossible with other methods. In the ongoing research, optical elastography, in combination with the traditional tensile test and tissue histology, will be used to characterize localized biomechanical properties directly within the defect area and to locate “crack” initiation and propagation sights as the material is strained to rupture.
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e-mail: hmarie@ysu.edu
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July 2010
Technical Briefs
Biomechanical and Elastographic Analysis of Mesenchymal Stromal Cell Treated Tissue Following Surgery
Hazel Marie,
Hazel Marie
Department of Mechanical and Industrial Engineering,
e-mail: hmarie@ysu.edu
Youngstown State University
, Youngstown, OH 44555
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Yong Zhang,
Yong Zhang
Department of Computer and Information Science,
Youngstown State University
, Youngstown, OH 44555
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Jeremy Heffner,
Jeremy Heffner
Department of General Surgery,
St. Elizabeth Health Partners
, Youngstown, OH 44504
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Heath A. Dorion,
Heath A. Dorion
Department of Education, and Department of General Surgery,
St. Elizabeth Health Partners
, Youngstown, OH 44504
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Diana L. Fagan
Diana L. Fagan
Department of Biological Sciences,
Youngstown State University
, Youngstown, OH 44555
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Hazel Marie
Department of Mechanical and Industrial Engineering,
Youngstown State University
, Youngstown, OH 44555e-mail: hmarie@ysu.edu
Yong Zhang
Department of Computer and Information Science,
Youngstown State University
, Youngstown, OH 44555
Jeremy Heffner
Department of General Surgery,
St. Elizabeth Health Partners
, Youngstown, OH 44504
Heath A. Dorion
Department of Education, and Department of General Surgery,
St. Elizabeth Health Partners
, Youngstown, OH 44504
Diana L. Fagan
Department of Biological Sciences,
Youngstown State University
, Youngstown, OH 44555J Biomech Eng. Jul 2010, 132(7): 074503 (4 pages)
Published Online: June 2, 2010
Article history
Received:
November 10, 2009
Revised:
February 25, 2010
Posted:
March 8, 2010
Published:
June 2, 2010
Online:
June 2, 2010
Citation
Marie, H., Zhang, Y., Heffner, J., Dorion, H. A., and Fagan, D. L. (June 2, 2010). "Biomechanical and Elastographic Analysis of Mesenchymal Stromal Cell Treated Tissue Following Surgery." ASME. J Biomech Eng. July 2010; 132(7): 074503. https://doi.org/10.1115/1.4001382
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