Mechanical deformation of cell-seeded electrospun matrices plays an important role in cell signaling. However, electrospun biomaterials have inherently complex geometries due to the random deposition of fibers during the electrospinning process. This confounds attempts at quantifying strains exerted on adherent cells during electrospun matrix deformation. We have developed a novel mechanical test platform that allows deposition and tensile testing of electrospun fibers in a highly parallel arrangement to simplify mechanical analysis of the fibers alone and with adherent cells. The device is capable of optically recording fiber strain in a cell culture environment. Here we report on the mechanical and viscoelastic properties of highly parallel electrospun poly(ε-caprolactone) fibers. Force-strain data derived from this device will drive the development of cellular mechanotransduction studies as well as the customization of electrospun matrices for specific engineered tissue applications.
A Novel Device to Quantify the Mechanical Properties of Electrospun Nanofibers
Fee, T. J., Dean, D. R., Eberhardt, A. W., and Berry, J. L. (October 1, 2012). "A Novel Device to Quantify the Mechanical Properties of Electrospun Nanofibers." ASME. J Biomech Eng. October 2012; 134(10): 104503. https://doi.org/10.1115/1.4007635
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